melanoma tissue microarray analysis (tma) slides (Biomax Inc)
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Melanoma Tissue Microarray Analysis (Tma) Slides, supplied by Biomax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1)"
Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1)
Journal: Scientific Reports
doi: 10.1038/s41598-017-14764-4
Figure Legend Snippet: The consistency of YAP and LRP1 in tissue microarray specimen. ( a , b ) TMA slides include forty skin melanoma tissues and eight skin normal tissues which locate on the bottom of the each TMA. Representative images of IHC from HCC TMA stained with anti-YAP or anti-LRP1 antibodies. Scale bar, 100 μM. ( c ) Representative images of IHC from skin melanoma HCC TMA stained with anti-YAP or anti-LRP1 antibodies. Scale bar, 100 μM. ( d ) The statistical figure of skin melanoma IHC images from HCC TMA stained with anti-YAP or anti-LRP1 antibodies. The TMA data were analyzed using the χ2 test.
Techniques Used: Microarray, Staining
Related Articles
Immunohistochemistry:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Microarray:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Staining:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Expressing:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Mutagenesis:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. CRISPR:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Sequencing:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Amplification:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Soft Agar Assay:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Imaging:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Western Blot:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Clone Assay:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Inhibition:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. Two Tailed Test:Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Article Snippet: For IHC, Article Title: Inhibition of Glycogen Metabolism Induces Reactive Oxygen Species-Dependent Cytotoxicity in Anaplastic Thyroid Cancer in Female Mice Article Snippet: Cooled slides were washed 3 times in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch, West Grove PA, USA) diluted in 5% bovine serum albumin (BSA) in PBS with 0.3% Triton X-100. Article Title: PVT1 dependence in cancer with MYC copy-number increase Article Snippet: Complementary DNA (cDNA) was synthesized from equal amounts of total RNA and followed the manufacturer’s protocol using SuperScript III reverse transcriptase (Invitrogen) with Random Hexamer primers. |